R2A Agar is a ready-to-use, low-nutrient solid medium commonly used for detecting heterotrophic bacteria in treated water. It is supplied in pre-sterilized glass bottles, eliminating the need for autoclaving or additional sterilization. This convenient format makes it ideal for laboratories working under strict time or contamination constraints.

Composition and Benefits of R2A Agar

• Casein acid hydrolysate, proteose peptone, and yeast extract provide essential nutrients like nitrogen, amino acids, carbon sources, and vitamins.

• Dextrose acts as a primary energy source for microbial metabolism.

• Soluble starch helps detoxify the medium by absorbing harmful metabolic byproducts, aiding in the recovery of sensitive organisms.

• Sodium pyruvate enhances the growth of stressed cells.

• Magnesium sulphate supplies essential divalent cations.

• Dipotassium phosphate maintains the pH balance of the medium.

Usage Guidelines

Melting and Dispensing Instructions

To prepare R2A Agar for use:

1. Slightly loosen the bottle cap.

2. Melt the medium in a water bath or other heat source until fully liquefied.

3. Pour the melted medium into sterile petri dishes or containers.

4. Allow it to cool and solidify before inoculating the sample.

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